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You are here:Home » Antibodies » Abs to Neurofilament » Anti -NES (Nestin, Nbla00170)

Anti -NES (Nestin, Nbla00170)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC FC
Nestin (NES) is an intermediate filament protein. Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Catalog #N2060-05E
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunocytochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2C1.3A11
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenA 150aa fragment from the cloned human Nestin conjugated to GST.
SpecificityRecognizes human Nestin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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