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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH IC FC

Nestin (NES) is an intermediate filament protein. Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Catalog #	N2060-05E
Applications	Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:5000
	Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
	Immunocytochemistry: 1:100-1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2C1.3A11
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	A 150aa fragment from the cloned human Nestin conjugated to GST.
Specificity	Recognizes human Nestin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.