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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Nestin

Anti -Nestin

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified B IH IC
Mouse Nestin is an approximately 207kD protein (1864aa) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.
Catalog #N2060-08
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde-fixed tissues.
Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde-fixed cells.
Western Blot: 1:2000-1:5000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceMouse
Concentration~0.3mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenThree synthetic peptides corresponding to different regions of the mouse Nestin (NP_057910) (KLH). Species Sequence Homology: rat (NM_012987, NCBI).
SpecificityRecognizes mouse Nestin. Note that the sequences of the mouse and human Nestin genes are divergent, and it is unlikely that antibodies raised against the mouse sequence would recognize human Nestin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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