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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes) (Biotin)

Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Nestin is a 240kD class VI intermediate filament protein that was first identified in neuroepithelial stem cells. It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% aa sequence homology with the mouse protein.
Catalog #N2060-10B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived, recombinant rat Nestin (rrNestin; aa544-776).
SpecificityRecognizes rat Nestin. Species sequence homology: mouse (76%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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