Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH IF

Neurofilaments (NFs) are prominent components of large myelinated axons. Based on sequence homology and intron placement, the NF genes have been classified as type IV IFs along with alpha-internexin, which is also expressed in the nervous system. NFs may function as effector proteins subject to a complex regulatory cross-talk between neurons and myelinating Schwann cells or oligrodendrocytes. Mammalian neurofilaments are assembled from light (NF-L), mid-sized (NF-M), and heavy (NF-H) neurofilament proteins. NF-M is required if myelinated axons are to achieve maximal diameters and NF-M has been shown to regulate the level of NF-L. NF-M subunits mainly regulate axonal caliber by altering the level of NF-L and in turn the number of NFs in the axon. Results seem to explain as a perturbation of NF stoichiometry in which overexpression of NF-M leads to the formation of perikaryal NF inclusions in motor neurons and the depletion of NFs from axons. Overexpression of NF-M in combination with NF-L increased radial growth of axons, which suggests that NF-M or NF-H must coassemble into filaments with NF-L before they can affect the radial growth of axons. The immature form of NFs composed of NF-L and NF-M, might function in establishing the early neuronal phenotype and in maintaining neurite outgrowth.
Catalog #	N2159-50
Applications	Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence: 5-10ug/ml
	ELISA: 0.1-1ug/ml
	Western Blot: 1-5ug/ml
	Immunohistochemistry (FFPE & Frozen Sections): 10ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3G201
Host	Mouse
Source	Rat
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Rat NF-M
Specificity	Recognizes rat NF-M. Species Crossreactivity: Human, mouse, chicken and rabbit.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.