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Abs to Neurofilament
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Anti -Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm)
Anti -Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Highly Purified |
B IH |
|
| Neurofilament proteins consist of the light NF-L, medium NF-M and heavy NF-H subunits.Most neurons appear to express these proteins but their ratios and phosphorylation states are variable. | | | Catalog # | N2159-50A | | Applications | Suitable for use in Immunohistochemistry (frozen) and Western Blot. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (frozen): 1:50-1:1,000 | | Western Blot: 1:1,000-1:2,000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10F216 | | Host | Mouse | | Source | Rat | | Concentration | As reported | | Form | Supplied as a liquid, 0.09% sodium azide. | | Purity | Purified by Ion Exchange chromatography. | | Immunogen | Recombinant fusion protein RM:677-845 containing the last 169 aaof rat neurofilament (145 kD). Purified from E. coli inclusion bodies by Ion exchange chromatography. Corresponds to the rat NF-M KE segment (lysine glutamate) (ref.1) | | Specificity | Recognizes an epitope dependent on the last 84aa of rat NF-M (145kD). Species Crossreactivity: birds, mammals. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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