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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm)

Anti -Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M
Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified B IH
Neurofilament proteins consist of the light NF-L, medium NF-M and heavy NF-H subunits.Most neurons appear to express these proteins but their ratios and phosphorylation states are variable.
Catalog #N2159-50A
ApplicationsSuitable for use in Immunohistochemistry (frozen) and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen): 1:50-1:1,000
Western Blot: 1:1,000-1:2,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No10F216
HostMouse
SourceRat
ConcentrationNot determined
FormSupplied as a liquid, 0.09% sodium azide.
PurityPurified by Ion Exchange chromatography.
ImmunogenRecombinant fusion protein RM:677-845 containing the last 169 aaof rat neurofilament (145 kD). Purified from E. coli inclusion bodies by Ion exchange chromatography. Corresponds to the rat NF-M KE segment (lysine glutamate) (ref.1)
SpecificityRecognizes an epitope dependent on the last 84aa of rat NF-M (145kD). Species Crossreactivity: birds, mammals.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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