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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm)

Anti -Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M
Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
Neurofilaments are the intermediate filaments found in neuronal cells (reviewed in 1 and 2). They are composed of a combination of subunit proteins which typically includes the neurofilment triplet proteins, NF-L (68kD), NF-M (160kD), and NF-H (200kD). Neurofilaments may also include peripherin, alpha-internexin (NF66), nestin, and vimentin. Antibodies to the various neurofilament subunits are useful biomarkers; the filaments are abundant and biochemically stable. The expression of neurofilament proteins varies during neuronal development and differentiation and between between neuronal cell subtypes, and can be altered in pathological states. For example, accumulation of neurofilaments is observed during the progression of numerous neurological diseases.
Catalog #N2159-50C
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone NoNF-09
HostMouse
SourcePorcine
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPorcine NEFM
SpecificityRecognizes porcine NEFM. Species sequence homology: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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