| Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
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| Catalog # | N2159-51 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | |
| Peptide ELISA Titer: 1:128,000 |
| Western Blot: 0.03-0.1ug/ml detects ~170-200kD band observed in Mouse Brain lysates (calculated MW of 102kD according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. |
| Purity | Purified by peptide affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to C-QASHITVERKDYLK, from the internal region of human NEFM (NP_005373.2; NP_001099011.1). |
| Specificity | Recognizes NEFM. Species crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | neurofilament-3 (150 kD medium), neurofilament, medium polypeptide 150kDa, neurofilament triplet M protein, NFM, NF-M, NEF3, neurofilament, medium polypeptide, NEFM |
