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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IP IH IF

Neurofilaments are the intermediate filaments of neurons and their processes. They are protein triplets composed of three major subunits of MW 68kD, 155kD and 200kD. The antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained. Neurofilaments are expressed in tumors of neural origin, or displaying neuronal differentiation, such as neuroblastoma, medulloblastoma and retinoblastoma.
Catalog #	N2160
Applications	Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (formalin/paraffin): 1:50-1:100 for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues.
	Immunoprecipitation (Native verified): 10ul/mg protein lysate. (Use protein G).
	Western Blot: 1:50 for 2hrs at RT
	Optimal dilutions to be determined by the researcher.
Positive Control	IMR-5 cells, Brain
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	0.N.467
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid, 15mM sodium azide.
Purity	Supernatant
Immunogen	Neuro lament purified from human brain. Cellular Localization:
Cytoplasmic
Specificity	Recognizes the phosphorylated forms (68kD and 200kD subunits) of human Neurofilament. Highly specific and shows no crossreactivity with other intermediate filament proteins. Species Crossreactivity: rabbit, feline, mouse and rat. Does not react with canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.