Anti -Neuro lament L, 68kD (Neurofilament Light Polypeptide, NF-L, Neurofilament Triplet L
Protein, 68kD Neurofilament Protein, Micro Glutamic Acid-Rich Protein, NEFL)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH IC IF |
|
| Neurofilaments are the intermediate filaments found in neuronal cells. They are composed of a combination of subunit proteins which typically includes the neurofilment triplet proteins, NF-L (68kD), NF-M (160kD), and NF-H (200kD). Neurofilaments may also include peripherin, alpha-internexin (NF66), nestin, and vimentin. Antibodies to the various neurofilament subunits are useful biomarkers; the filaments are abundant and biochemically stable. The expression of neurofilament proteins varies during neuronal development and differentiation and between neuronal cell subtypes. Expression can be altered in pathological states. For example, accumulation of neurofilaments is observed during the progression of numerous neurological diseases. Neurofilament NF-L/ A Neural Progenitors cell type. Important Structural protein expressed in primitive neural tissue. Present in NT2 cells. | | | Catalog # | N2160-01A | | Applications | Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:100-1:500 | | Western Blot: 1:500-1:2500 | | Immunohistochemistry: 1:100-1:500 | | Immunocytochemistry: 1:100-1:500 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Brain and spinal cord | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 11E290 | | Host | Mouse | | Source | Porcine | | Concentration | As reported | | Form | Supplied as a liquid, 0.01% thimerosal, 1mg/ml BSA, 50% glycerol. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. | | Specificity | Recognizes porcine NEFL. Species Crossreactivity: bovine, feline, chicken, human, mouse, rat. | | Species sequence homology | Avian, most mammals. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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