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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament Light (NF-L, NF68, NEFL, Neurofilament light polypeptide, NFL)

Anti -Neurofilament Light (NF-L, NF68, NEFL, Neurofilament light polypeptide, NFL)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #N2160-01B1
Neurofilaments are composed of three intermediate filament proteins light (68kD), medium (160kD) and heavy (200kD), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunohistochemistry (formalin, paraffin, acetone): 2.0-4.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G400
HostMouse
SourcePorcine
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
PurityPurified by affinity chromatography.
ImmunogenPorcine spinal cord.
SpecificityRecognizes dephosphorylated porcine NF68. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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