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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B IH

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease or Alzheimerís disease.
Catalog #	N2160-01E
Applications	Suitable for use in Immunohistochemistry, Western Blot. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:5000
	Immunohistochemistry (Cryostat sections and formalin-fixed paraffin-embedded sections): 1:1000.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Porcine
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Serum
Immunogen	Native NF-L purified from porcine spinal cord
Specificity	Recognizes the light neurofilament subunit (~68kD).
Species crossreactivity	Human, bovine, porcine, mouse, rat, avian. Should crossreact with most other mammals.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.