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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament L, 68kD (NF-L)

Anti -Neurofilament L, 68kD (NF-L)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease or Alzheimerís disease.
Catalog #N2160-01E
ApplicationsSuitable for use in Immunohistochemistry, Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:5000
Immunohistochemistry (Cryostat sections and formalin-fixed paraffin-embedded sections): 1:1000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourcePorcine
ConcentrationNot determined
FormSupplied as a liquid.
PuritySerum
ImmunogenNative NF-L purified from porcine spinal cord
SpecificityRecognizes the light neurofilament subunit (~68kD).
Species crossreactivityHuman, bovine, porcine, mouse, rat, avian. Should crossreact with most other mammals.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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