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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neuro lament L, 68kD (NF-L)

Anti -Neuro lament L, 68kD (NF-L)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #N2160-01J
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects neurofilament, light chain in RIPA lysates from a rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Neurofilament, light chain (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry Reported by independent laboratories to detect neurofilament, light chain in frozen human Alzheimer’s brain sections and rat brain sections (2,3).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4H359
HostMouse
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified and enzymatically dephosphorylated porcine neurofilament, light chain.
SpecificityRecognizes neurofilament, light chain, Mr 68kD and a protein fragment, Mr 56kD. Species Crossreactivity: Human, porcine, mouse, rat, bovine, avian and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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