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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament L, 68kD (NF-L)

Anti -Neurofilament L, 68kD (NF-L)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Neurofilaments are the 10 nm or intermediate filament proteins found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed predominantly of three major proteins called NFL, NFM and NFH. NFL is the neurofilament light or low molecular weight polypeptide and runs on SDS PAGE gels at about 68,000 Da. Antibodies to NFL are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NFL antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. Mutations in the protein coding region of the human NFL gene appear to cause some forms of Charcot Marie Tooth disease.
Catalog #N2160-01L1
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:50-1:250
Western Blot: 1:2500-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No8A224
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide
PurityPurified by immunoaffinity chromatography.
ImmunogenNeurofilament Neurofilament L
SpecificityRecognizes Neurofilament L. Species Crossreactivity: human, rat, mouse, feline and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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