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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IC IF

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Catalog #	N2160-01L2
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Immunofluorescence: 1:250-1:500
	Western Blot: 1:10,000
	Immunocytochemistry (ABC): 1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Porcine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Porcine intermediate filaments were prepared from spinal cords by the method of Delacourte et al., and dissolved in 6M urea.
Specificity	Recognizes porcine NF-L. Species Crossreactivity: human, rat, mouse, feline and chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.