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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament L, 68kD (NF-L)

Anti -Neurofilament L, 68kD (NF-L)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200kD), NF-M the medium (160kD) and NF-L the light protein (68kD) (2). NF-L self polymerizes and provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M (3). NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons (4).
Catalog #N2160-01L3
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1,000-1:50,000
Flow cytometry: 1:70
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E396
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the C- term of human NF-L.
SpecificityRecognizes human Neurofilament L, CT. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesNF-M, Neurofilament triplet M protein, 160kD neurofilament protein, Neurofilament 3


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