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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP FC

Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200kD), NF-M the medium (160kD) and NF-L the light protein (68kD) (2). NF-L self polymerizes and provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M (3). NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons (4).
Catalog #	N2160-01L3
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:1,000-1:50,000
	Flow cytometry: 1:70
	Immunoprecipitation: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E396
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues near the C- term of human NF-L.
Specificity	Recognizes human Neurofilament L, CT. Species crossreactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	NF-M, Neurofilament triplet M protein, 160kD neurofilament protein, Neurofilament 3