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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament L, CT (68kD neurofilament protein, Neurofilament triplet L protein, light molecular weight neurofilament protein, neurofilament, light polypeptide 68kD)

Anti -Neurofilament L, CT (68kD neurofilament protein, Neurofilament triplet L protein,
light molecular weight neurofilament protein, neurofilament, light polypeptide 68kD)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complex of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure, however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
Catalog #N2160-01L4
ApplicationsSuitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:70
Western Blot: 1:50000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlSHSY5Y cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i181
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues near the C-terminus of human Neurofilament-L.
SpecificityRecognizes human Neurofilament-L at and around the C-terminus. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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