| Human neurofilament light chain (NF-L) is a 61.516 kD protein (543aa) expressed in the axons of CNS and PNS neurons, where it plays a role in maintaining the structural integrity of the axon. Mutations of the NF-L gene product are responsible for some forms of autosomal dominant Charcot-Marie-Tooth neuropathies in humans (i.e., CMT type 1F). |
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| Catalog # | N2160-01L5 |
| Applications | Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | |
| Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues |
| Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells |
| Western Blot: 1:2000-1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch |
| I1906-10 IgY, Chicken (HRP) Rb x Ch |
| I1906-12 IgY, Chicken (FITC) Rb x Ch |
| I1906-14 IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Mouse |
| Concentration | ~0.3mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Three synthetic peptides corresponding to different regions of the NF-L gene product (KLH). Species sequence homology: human (P07196, NCBI) and mouse (P08551, NCBI) |
| Specificity | Recognizes Neurofilament Light Chain. Species crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
