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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IP IH

Neurofilaments are intermediate (10–12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD), and NF-H (200kD). The neurofilaments are long helical proteins which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, phosphorylation likely mediates neurofilament dynamics. However, how O-linked N-acetylglucosamine residues function to modify neurofilament function remains unclear.
Catalog #	N2160-01N
Applications	Suitable for use in ELISA, Immunoblotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 0.1–0.5ug/ml
	Immunohistochemistry: 5–10ug/ml (paraffin-embedded or frozen sections)
	Immunoblotting: 0.5–1.0ug/ml
	Immunoprecipitation: 2–5ug
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3G202
Host	Mouse
Source	Rat
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Rat neurofilaments
Specificity	Reacts with the 160 kD polypeptides of human neurofilament. It specifically recognizes a phosphateindependent epitope in the tail (carboxy) domain of NF-M of most vertebrates and invertebrates.
Species crossreactivity	Human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.