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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IF

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160kD, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Catalog #	N2160-01R
Applications	Suitable for use in Immunofluorescence, Western Blotting. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:100
	Western Blotting: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6H9
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Tissue culture supernatant
Immunogen	Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli (1).
Specificity	The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species. Recognizes intact NF-M strongly and specifically on Western Blots in all mammals and birds tested to date and also binds NF-M in cells in tissue culture and in sectioned material.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.