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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IF

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145,000~170,000 Da, with some variation in different species. Antibodies to NF-M are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Catalog #	N2160-01T1
Applications	Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:50
	Western Blot: 1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Clone No	8A225
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli
Specificity	Recognizes NF-M from human, rat, mouse, cat, chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.