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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament M, 145-160kD (NF-M)

Anti -Neurofilament M, 145-160kD (NF-M)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145,000~170,000 Da, with some variation in different species. Antibodies to NF-M are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Catalog #N2160-01T1
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:50
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No8A225
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli
SpecificityRecognizes NF-M from human, rat, mouse, cat, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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