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Anti -Neurofilament M, 150kD, phosphorylated (NF-M)
Anti -Neurofilament M, 150kD, phosphorylated (NF-M)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B IH |
|
| Catalog # | N2160-02A | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:5000 detects phospho-neurofilament, medium chain in a rat brain microsomal preparation. Lambda phosphatase treated and untreated rat brain microsomal preparations were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-Neurofilament, medium chain (1:5000 dilution). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Immunohistochemistry | Detects phospho-neurofilament in rat embryo spinal cords (1). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Clone No | 4H360 | | Host | Mouse | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a liquid diluted 1:10 in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Ascites | | Immunogen | HPLC purified rat neurofilament isolated from rat spinal cords. | | Specificity | Recognizes phospho-neurofilament, medium chain, Mr 150kD. Species Cross-reactivity: Human and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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