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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament NF-M

Anti -Neurofilament NF-M

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Purified B IH IC
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
Catalog #N2160-02B
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:10,000
Immunohistochemistry: 1:2000
Immunocytochemistry: 1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
ConcentrationNot determined
FormSupplied as a liquid.
PurityPurified immunoglobulin
ImmunogenPolyclonal recombinant C-terminus (KE segment) of NF-M
SpecificityRecognizes NF-M. Species Crossreactivity: Feline, chicken, human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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