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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble.
Catalog #	N2160-02G
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:1000-1:2000
	Immunohistochemistry (frozen): 1:50-1:1000 4% PFA fixed tissue sections
	Optimal dilutions to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	2Q168
Host	Mouse
Source	Rat
Concentration	As reported
Form	Supplied as a liquid with no preservatives.
Purity	Supernatant
Immunogen	Purified recombinant fusion protein containing the last 168 amino acids of rat NF-M.
Specificity	Recognizes an epitope from the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.