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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament M,H 160-200 kD (NF-M+H)

Anti -Neurofilament M,H 160-200 kD (NF-M+H)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IH
Neurofilaments are intermediate (10–12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD), and NF-H (200kD). The neurofilaments are long helical proteins, which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, phosphorylation likely mediates neurofilament dynamics. However, how O-linked N-acetylglucosamine residues function to modify neurofilament function remains unclear.
Catalog #N2160-04L
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.1–0.5ug/ml
Immunohistochemistry (Bouin’s and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10ug/ml. To stain, incubate 30–60 minutes at RT or overnight at 4°C.
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 2-5ug
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G205
HostMouse
SourceRat
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPurified from mouse ascites fluid.
ImmunogenAdult rat neurofilament
SpecificityRecognizes the 160 & 200kD proteins of human neurofilament. Specifically recognizes a non-phosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody. Species Crossreactivity: human, rat, mouse, bovine, rabbit, hamster and squid.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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