| Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200kD) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70kD NF-protein can self-assemble into a filamentous structure, whereas the 160kD and 200kD NF-proteins require the presence of the 70kD NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in |
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| non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed |
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| together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation. |
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| Catalog # | N2160-04M |
| Applications | Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:50-1:100 |
| Immunohistochemistry: 1:50-1:100 |
| Western Blot: 1:100-1:500 |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 6G5 |
| Host | Mouse |
| Source | Bovine |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified |
| Immunogen | Neurofilament preparation of calf brain tissue. |
| Specificity | Reacts exclusively with the phosphorylated isoform of the 160kD neurofilament protein. |
| Species Reactivity | Human, rabbit, hamster, monkey, guinea pig and Xenopus. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
