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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.
Catalog #	N2160-05
Hybridoma	Derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5G17
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 1% BSA and 0.1% sodium azide.
Purity	Purified
Immunogen	Purified neurofilament triplet proteins from human spinal cord
Specificity	Recognizes the Mr 68kD and 200kD neurofilament proteins in human tissues.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.