Anti -Neurofilament heavy polypeptide (NF-200, NF200, NF-H, NEFH, N52, Neurofilament
triplet H protein, 200kD neurofilament protein, KIAA0845, NFH)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 Kd (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT). | | | Catalog # | N2160-06B | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunofluorescence. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5ml | | Immunohistochemistry (paraffin, frozen): 1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 10K289 | | Host | Mouse | | Concentration | As reported | | Form | Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. | | Specificity | Recognizes NF200. Species crossreactivity: human, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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