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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament heavy polypeptide (NF-200, NF200, NF-H, NEFH, N52, Neurofilament triplet H protein, 200kD neurofilament protein, KIAA0845, NFH)

Anti -Neurofilament heavy polypeptide (NF-200, NF200, NF-H, NEFH, N52, Neurofilament
triplet H protein, 200kD neurofilament protein, KIAA0845, NFH)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 Kd (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).
Catalog #N2160-06B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (FFPE): 1-2ug/ml
Immunohistochemistry (Frozen): 1-2ug/ml using formalin or acetone fixed tissues
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10K289
HostMouse
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenC-terminal segment of enzymatically dephosphorylated porcine Neurofilament 200.
SpecificityRecognizes Neurofilament 200. Species Crossreactivity: human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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