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Abs to Neurofilament
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Anti -Neurofilament heavy polypeptide (NF-200, NF200, NF-H, NEFH, N52, Neurofilament triplet H protein, 200kD neurofilament protein, KIAA0845, NFH)
Anti -Neurofilament heavy polypeptide (NF-200, NF200, NF-H, NEFH, N52, Neurofilament
triplet H protein, 200kD neurofilament protein, KIAA0845, NFH)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH |
|
| Catalog # | N2160-06B1 | | Neurofilaments contain three intermediate filament proteins | light (68kD), medium (160kD) and heavy (200kD). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5ug/ml | | Immunohistochemistry (formalin,paraffin, acetone): 1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9G401 | | Host | Mouse | | Source | Porcine | | Concentration | As reported | | Form | Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml. | | Purity | Purified by affinity chromatography. | | Immunogen | C-terminal segment of enzymatically dephosphorylated porcine Neurofilament Heavy (NF200). | | Specificity | Recognizes both phosphorylated and dephosphorylated forms of NF200/NF-H. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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