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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H, 180-220kD (NF-H)

Anti -Neurofilament H, 180-220kD (NF-H)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified B IH
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Catalog #N2160-06E
ApplicationsSuitable for use in Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern blot: 510ug/ml
Immunohistochemistry: 510ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q165
HostMouse
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified immunoglobulin (Ion exchange chromatography)
ImmunogenCarboxy terminal tail segment of enzymatically dephosphorylated porcine Hchain.
SpecificityRecognizes the phophorylated and dephosphorylated H-chain of neurofilament 200kD. It is used for the detection of cells of neuronal origin and Western blot. Species Reactivity: Human, monkey, porcine and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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