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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H, 180-220kD (NF-H) )

Anti -Neurofilament H, 180-220kD (NF-H) )

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160 and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure, however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
Catalog #N2160-06H
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 5ug/ml. Optimal staining with citrate buffer, pH 6.0.
Immunohistochemistry: Acetone-fixed frozen sections
Western Blot: 1:500 detected Neurofilament H on 10ug of mouse brain membrance lysates.
Optimal dilutions to be determined by researcher.
Positive ControlBrain; Cultured neurons
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q188
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRat brain protein preparation
SpecificityRecognizes rat Neurofilament H. Reacts with an epitope present on human neurofilaments 200kD and both bovine 160kD and 200kD neurofilaments. Reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type). Species Crossreactivity: human, mouse, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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