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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H, 180-220kD (NF-H) )

Anti -Neurofilament H, 180-220kD (NF-H) )

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites IH IC
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160 and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure. The 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung express neurofilaments.
Catalog #N2160-06J
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No2Q189
HostMouse
SourceRat
ConcentrationNot determined
FormAscites fluid, no preservatives
PurityAscites
ImmunogenRecombinant fusion protein containing the C-terminal 226 amino acids of rat NF-H.
SpecificityStrong reactivity on rat NF-H (200kD), somewhat weaker on other mammalian species. Does not recognize avian NF-H. Staining is not affected by the level of NF-H phosphorylation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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