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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament, heavy chain, 180-220kD (NF-H)

Anti -Neurofilament, heavy chain, 180-220kD (NF-H)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH IF
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease or Alzheimerís disease.
Catalog #N2160-06L
Application(s) Immunohistochemistry, Western Blot.
Recommended DilutionWestern Blot 1:5000
Immunohistochemistry 1:1000. Cryostat sections and formalin-fixed paraffin-embedded sections
Immunofluorescence (1:1,000)
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as serum, 0.1% sodium azide.
PuritySerum
ImmunogenRecombinant C terminal extension of rat NF-H
SpecificityRecognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220kD). Species crossreactivity: Human, feline, rat, bovine, mouse and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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