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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament, heavy chain, 180-220kD (NF-H)

Anti -Neurofilament, heavy chain, 180-220kD (NF-H)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight neurofilament subunit and runs on SDS-PAGE gels in the range 180-220kD with some variation in different species. This monoclonal recognizes specifically the phosphorylated form of NF-H which is normally found only in axons in the adult nervous system. Since closely related phosphorylated epitopes are also found on NF-M, this antibody shows some reactivity with phosphorylated forms of this protein also. Interestingly, in many damage and disease states the phosphorylated form of NF-H accumulate in the perikarya of neurons. The antibody can be used to identify axons in tissue sections and in tissue culture. It can also be used to study phosphorylated neurofilament accumulations seen in many neurological diseases and following neuronal injury.
Catalog #N2160-06M
Can be used on cryostat sections and on formalin-fixed cells in tissue culture. The antibody works well in ELISA and Western Blot, and is particularly appropriate for paraffin-embedded histological sections.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3C163
HostMouse
SourcePorcine
ConcentrationNot determined
FormSupplied as ascites, 0.01M PBS, pH7.6, 0.05% sodium azide, 0.01% Thimerosal, 15mg/ml BSA
PurityAscities
ImmunogenNative pig spinal cord neurofilaments
SpecificityRecognizes the phosphorylated variant of NF-H subunit (~180-220kD), showing some weaker reactivity with phosphorylated forms of NF-M. Species crossreactivity: Human NF-H and all other mammals tested to date. Also recognizes avian NF-H.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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