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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neuro lament L,H, 70kD, 200kD (NF-L+H)

Anti -Neuro lament L,H, 70kD, 200kD (NF-L+H)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IP IH IC IF
Neurofilaments (NFs), the intermediate filaments (IFs) specific to neurons, are the predominant cytoskeletal elements in many nerve cells. They are believed not only to confer mechanical stability, but also to play a role in determining axonal caliber. Human NFs are composed of three protein subunits, NF-L,NF-M and NF-H, which have mol. wts predicted from their gene sequences of-62,000,-102,000 and-110,000 respectively. Each type of NFs contains a central a-helical 'rod' domain of-310aa, characterized by a heptad repeat in the aa sequence in which the first and fourth residues are apolar. Perikaryal NFs are predominantly unphosphorylated, while axonal NFs are highly phosphorylated. Defects in NFL are the cause of Charcot-Marie-Tooth disease type 1F while defects in NFH are a cause of susceptibility to amyotrophic lateral sclerosis. The gene bank accession no of NF-L, NF-M and NF-H are NP_006149.1, NP_066554.2 and NP_005373.1 respectively.
Catalog #N2160-08B
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:50-1:100
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlBrain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No11E292
HostMouse
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid, 0.05% sodium azide.
PuritySupernatant
ImmunogenPurified neurofilaments from human brain. Cellular Localization: Cytoplasmic.
SpecificityRecognizes the 70 and 200kD component of human Neurofilaments. It stains neurons, neuronal processes and peripheral nerves. This antibody reacts with some neuroblastomas and gangliomas but not with other non-neuronal tumors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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