Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H, 200kD (NF-H)

Anti -Neurofilament H, 200kD (NF-H)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified B IH
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Catalog #N2160-14
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: MAP1B and Tau
Immunohistochemistry (Frozen, Paraffin): 1:50-1:100
Note: Does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Recommended Secondary ReagentsGoat anti-mouse IgG: HRP conjugate (rat absorbed), F(ab')2 rabbit anti-mouse IgG HRP conjugate
Recommended Positive Control TissueBrain or spinal cord
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2769
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PurityPurified by hydrophobic interaction chromatography.
ImmunogenNeurofilament H, 200kD
SpecificityRecognizes the 200kD neurofilament protein in a range of species.
Species CrossreactivityMouse, human, rat, porcine, chicken and reptile.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links