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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H, 200kD (NF-H)

Anti -Neurofilament H, 200kD (NF-H)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
Neurofilaments can be defined as the intermediate or 10 nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10 nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Catalog #N2160-14B
ApplicationsELISA
Immunohistochemistry: (frozen)- 1:500, (paraffin)- 1:500
Western Blotting: 1:500
Optimal working dilutions to be determined by researcher.
Immunohistochemistry This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Recommended Secondary Reagents Sheep anti Rabbit IgG: HRP
Recommended Positive Control Tissue Brain or Spinal Cord
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
ConcentrationNot determined
FormWhole serum. Supplied as a liquid with 0.1% sodium azide.
PurityNeat Serum.
ImmunogenHighly purified bovine neurofilament proteins.
SpecificityReacts with 200kD neurofilament H and shows less than 1% crossreactivity with NF-L and NF-M. In Immunohistochemistry the antiserum stains thick and thin axons and also faint staining of occasional cell bodies. Species Crossreactivity: Reactive with rat, bovine and human neurofilaments
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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