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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH IF

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kD, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyothrophic lateral sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. NAP4 is one of numerous antibodies which reacts
preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with NAP4 in dendritic and perikaryal neurofilaments.
Catalog #	N2160-14H
Applications	Suitable for use in Immunofluorescence, Western Blotting, Immunohistochemistry. Other
	applications not tested.
Recommended Dilution	Immunofluorescence: 1:500
	Western Blotting: 1:10000
	Immunohistochemistry: 1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6H7
Host	Mouse
Source	Porcine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Tissue culture supernatant
Immunogen	Native NF-H purified from porcine spinal cord.
Specificity	Recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. Recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.