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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H, 200-220kD (NF-H)

Anti -Neurofilament H, 200-220kD (NF-H)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IF
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kD, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyothrophic lateral sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. NAP4 is one of numerous antibodies which reacts
preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with NAP4 in dendritic and perikaryal neurofilaments.
Catalog #N2160-14H
ApplicationsSuitable for use in Immunofluorescence, Western Blotting, Immunohistochemistry. Other
applications not tested.
Recommended DilutionImmunofluorescence: 1:500
Western Blotting: 1:10000
Immunohistochemistry: 1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6H7
HostMouse
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityTissue culture supernatant
ImmunogenNative NF-H purified from porcine spinal cord.
SpecificityRecognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. Recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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