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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H, 200kD (NF-H)

Anti -Neurofilament H, 200kD (NF-H)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IH
Neurofilaments are intermediate (10–12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD). And NF-H (200kD). The neurofilaments are long helical proteins which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, phosphorylation likely mediates neurofilament function remains unclear.
Catalog #N2160-16
Cloning partner Sp/2
Neurofilament proteins (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal axons throughout the central and peripheral nervous system. Since NFPs are major structural proteins and biochemically quite stable, antibodies to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original cell type of the tumor. In addition, the normal NFP staining pattern is altered in a variety of human diseases including Alzheimer’s disease, diffuse cortical Lewy body disease, Parkinson’s disease and amyotrophic lateral sclerosis (Lou Gehrig’ disease). Alterations in the NFP expression pattern are also seen in most toxin-induced, sporadic and heriditary axonopathies ocurring in humans and animals.
ApplicationsSuitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunoblotting. Other applications not tested.
Recommended DilutionELISA: 0.1–0.5ug/ml
Western Blotting: 1:10-1:50
Immunohistochemistry: 5–10ug/ml (paraffin-embedded sections)
Immunoblotting: 0.5–1.0ug/ml
Immunoprecipitation: 2–5ug
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G206
HostMouse
SourceRat
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurifeid from mouse ascites fluid.
ImmunogenRat neurofilaments
SpecificityReacts with the 200 kD polypeptides of human neurofilament. It specifically recognizes a phosphate-dependent epitope in the tail domain of NF-H. Dephosphorylation of the NF-H will diminish the immunoreactivity of RMO-24.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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