| Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. |
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| Catalog # | N2160-16A |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:100,000 |
| Immunohistochemistry: 1:100,000 |
| Immunocytochemistry: 1:100,000 |
| IOptimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1903-80: IgY, H&L, X-Adsorbed, 12nm (Gold) Pab Dk x Ch |
| I1903-80A: IgY, H&L, X-Adsorbed, 18nm (Gold) Pab Dk x Ch |
| I1903-80B: IgY, H&L, X-Adsorbed, 6nm (Gold) Pab Dk x Ch |
| I1906: IgY Pab Rb x Ch |
| I1906-01: IgY, Fc (HRP) Pab Gt x Ch |
| I1906-10: IgY (HRP) Pab Rb x Ch |
| I1906-12: IgY (FITC) Pab Rb x Ch |
| I1906-14: IgY (AP) Pab Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Bovine |
| Concentration | Not determined |
| Form | Supplied as a liquid. |
| Purity | Serum |
| Immunogen | Bovine intermediate filaments from spinal cords |
| Specificity | Recognizes bovine NF-H. Species Crossreactivity: Human, mouse, rat and primate. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
