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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament H (neurofilament, heavy polypeptide, 200kD neurofilament protein, KIAA0845, Neurofilament heavy polypeptide, Neurofilament triplet H protein, NFH, NF-H) (Alexa FluorŪ488)

Anti -Neurofilament H (neurofilament, heavy polypeptide, 200kD neurofilament protein,
KIAA0845, Neurofilament heavy polypeptide, Neurofilament triplet H protein, NFH, NF-H)
(Alexa FluorŪ488)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure; however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
Catalog #N2160-16B
ApplicationsSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen): 5-10ug/ml
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.
Positive ControlRat Cortex Cells.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Alexa Fluor®488 is light sensitive.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H258
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M phosphate buffer, 0.25M sodium chloride, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified neurofilament polypeptides (Debus et al., 1983).
SpecificityRecognizes rat, human, and pig Neurofilament H.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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