Anti -Neurofilament H (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H
Protein, 200kD Neurofilament Protein, Nefh, Nfh)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IC IF |
|
| Neurofilaments are the intermediate filaments found in neuronal cells. They are composed of a combination of subunit proteins which typically includes the neurofilment triplet proteins, NF-L (68kD), NF-M (160kD), and NF-H (200kD). Neurofilaments may also include peripherin, alpha-internexin (NF66), nestin, and vimentin. Neurofilament proteins are useful biomarkers as their expression can vary during neuronal development and differentiation and between between neuronal cell subtypes, and altered in pathological states. Accumulation of neurofilaments is observed the progression of numerous neurological diseases. NF-H, the heavy molecular weight neurofilment protein, is phosphorylated on KSP (lysine-serine-proline) repeats found in the C-terminal tail domain. The NF-H tail domain contains 42-51 KSP repeats and most of these sites are normally phosphorylated in vivo. However, the level of phosphorylation and the ratio between phosphorylated and unphosphorylated NF-H can vary and can also be altered during pathological states. Mutations in the NF-H tail domain have been linked to some forms of amyotropic lateral sclerosis (ALS), a progressive motor neuron degenerative disease resulting in paralysis and death. Antibodies to NF-H are useful for identifying and analyzing neuronal cells and their processes in tissue culture and in tissue sections. | | | Catalog # | N2160-16E | | Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:100-1:500 | | Western Blot: 1:1000-1:2500 | | Immunocytochemistry: 1:100-1:500 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Brain or spinal cord | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Bovine | | Concentration | As reported | | Form | Supplied as a liquid, 0.01% thimerosal, 1mg/ml BSA, 50% glycerol. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Bovine neurofilament NF-H. | | Specificity | Recognizes Neurofilament H. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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