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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neuro lament, Subunit tail, Heavy (NF-H)

Anti -Neuro lament, Subunit tail, Heavy (NF-H)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The human heavy neuro lament subunit (NFH) tail that contains a repeated motif, XKSPYK. Deletions in this repeat region are strongly implicated as a primary event in amyotrophic lateral sclerosis.
Catalog #N2160-35
Neurofilaments can be defined as the intermediate or 10 nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10 nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide before the addition of 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenXKSPYK domain [SPEKAKSPEKAKSC ] of NFH.
SpecificityReacts with the repeated motif, XKSPYK domain [SPEKAKSPEKAKSC ] of NFH.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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