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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament M, 160kD (NF-M, NFM, 150kD Medium, 160kD Neurofilament Protein, Neurofilament 3, NEF3, Neurofilament Medium Polypeptide, Neurofilament Protein Medium, NEFM, Neurofilament Triplet M Protein, NF160, NF165)

Anti -Neurofilament M, 160kD (NF-M, NFM, 150kD Medium, 160kD Neurofilament Protein,
Neurofilament 3, NEF3, Neurofilament Medium Polypeptide, Neurofilament Protein Medium,
NEFM, Neurofilament Triplet M Protein, NF160, NF165)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IC
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H.
Catalog #N2165-11C
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry: frozen and paraffin sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9A24
HostMouse
SourcePorcine
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPellet of pig brain cold stable proteins after depolymerization of microtubules.
SpecificityRecognizes both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160kD) of various species. Species Crossreactivity: all Mammals, a wide range of other species, human, rat, mouse, bovine and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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