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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IH

Catalog #	N2165-12
Recommended Secondary Reagents	Sheep anti Rabbit IgG: HRP
Recommended Positive Control Tissue	Brain or Spinal cord
Applications	ELISA
	Immunohistochemistry: (frozen)- 1:500, (paraffin)- 1:500
	Western Blotting: 1:500
	Optimal working dilutions to be determined by researcher.
Immunohistochemistry	* Glutaraldehyde fixation is not recommended.This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Bovine
Concentration	Not determined
Form	Serum. Supplied as a liquid with 0.1% sodium azide.
Purity	Serum.
Immunogen	Highly purified bovine neurofilament proteins.
Specificity	Reacts with 150kD neurofilament M and shows less than 2% crossreactivity with NF-L and approximately 3% with NF-H. In Immunohistochemistry the antiserum stains neurons and cell bodies throughout the central and peripheral nervous system. Species Crossreactivity: Rat, bovine and human neurofilaments
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.