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Specifications

Clone Host Grade Applications Polyclonal Chicken Affinity Purified IH

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
Catalog #	N2165-15
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 1:50 -1:100
	Optimal dilutions to be determined by the researcher.
Positive Control	Neonatal rat cortical neurons
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgY
Host	Chicken
Concentration	Not Determined
Form	Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide
Purity	Purified by affinity chromatography.
Immunogen	Recombinant protein expressed in bacteria
Specificity	Recognizes Neurofilament, M (NF-M).
Species Crossreactivity	Human and mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.