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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Neurogranin, NRGN (Protein Kinase C Substrate, RC3)

Anti -Neurogranin, NRGN (Protein Kinase C Substrate, RC3)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.
Catalog #N2171-15
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:8000
Western Blot: 0.1-0.3ug/ml detects ~15kD band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6kD according to NP_006167.1). There is also an additional 16kD band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300;
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to C-KIKSGERGRKG, from the internal region of human Neurogranin (NP_006167.1).
SpecificityRecognizes human Neurogranin. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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