Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E

The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction. Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction). NmU-like immunoreactivity has been detected in mammalian brain, GI-tracts of various species, and in the thyroid and endocrine cells of pituitary glands. Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission. Recently two structurally related, orphan G-protein coupled receptors, termed NMUR1 and NMUR2 have been identified as cognate receptors of NmU.
NmU is synthesized from a large precursor peptide and cleaved into 25aa (human, 25aa; rat, 23aa; porcine, 25aa) and 8aa (Nmu-8; 18-25) biologically active peptides. NMU peptides from various species share the greatest homology in the their C-terminal regions, which is also critical in biological activity. NMU is present in nerves throughout the GI-tracts, corticotrophs within the anterior and lobe of rat and human pituitary glands, parafollicular cells of in rat thyroid gland, and in various regions of brain (spinal cord, hypothalamus, substantia nigra, hippocampus, amygdala). Low levels of NmU are also found in human adipose tissue, lymphocytes, and spleen.
Catalog #	N2171-80H1
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	ELISA: 1:100,000 using 50-100ng of control peptide/well. See N2171-80G.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute in 100ul sterile PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Three peptides from human, mouse and rat Neuromedin U (NmU), within the N-terminal domain conjugated to KLH.
Specificity	Recognizes Neuromedin U. Species Crossreactivity: human, mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.