| The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction. Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction). NmU-like immunoreactivity has been detected in mammalian brain, GI-tracts of various species, and in the thyroid and endocrine cells of pituitary glands. Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission. Recently two structurally related, orphan G-protein coupled receptors, termed NMUR1 (GPCR66/FM-3/SNORF62) and NMUR2 (TGR-1/FM-4/SNORF72), have been identified as cognate receptors of NmU. |
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| Catalog # | N2171-80P |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1-10ug/ml using ECL. |
| ELISA: ~1:100,000 using 50-100ng control peptide/well. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | N2171-80N: Neuromedin U, Mouse Control Peptide (NmU) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to 14aa within the N-terminal of mouse NmU conjugated to KLH. |
| Specificity | Recognizes mouse Neuromedin U. No significant sequence homology with rat or human or NmU from other species. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
