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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron Specific Enolase (NSE)

Anti -Neuron Specific Enolase (NSE)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2-phospho-glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (a, b and g). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
Catalog #N2175-01B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended DilutionsWestern Blot: 2-4ug/ml for 2 hrs at RT
Immunohistology: (Formalin/paraffin) 1:100 to 1:150 for 30 min) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control IMR-5 cells, Pancreas
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%. .
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from C-terminal of human NSE. Cellular Localization: Cytoplasmic.
SpecificityRecognizes human NSE, Mr 46kD
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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