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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron Specific Enolase (NSE)

Anti -Neuron Specific Enolase (NSE)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified B IH IC
Human NSE-2 (EC 4.2.1.11) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-Dglycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.
Catalog #N2175-01D
ApplicationsSuitable for use in RWestern Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:250-1:500
Immunohistochemistry: 1:100-1:200
Immunocytochemistry: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponded to a region of the NSE gene product shared between the rat (AAA41119, NCBI) and human (NP_001966, NCBI) gene products.
SpecificityRecognizes human and rat NSE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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